Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements.

نویسندگان

  • Takakazu Nakabayashi
  • Hui-Ping Wang
  • Masataka Kinjo
  • Nobuhiro Ohta
چکیده

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

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عنوان ژورنال:
  • Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

دوره 7 6  شماره 

صفحات  -

تاریخ انتشار 2008